CD83 Rekombinanter Antikörper

CD83 Rekombinant Antikörper für IF/ICC, FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

IF/ICC, FC

Konjugation

Unkonjugiert

CloneNo.

HB15e

Kat-Nr. : 65581-1-MR

Synonyme

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

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  IF Staining of Raji using 65581-1-MR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD83 antibody (65581-1-MR, Clone: HB15e ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of human monocyte-derived mature dendritic cells using 65581-1-MR

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human CD83 Mouse IgG2a Recombinant Antibody (65581-1-MR, Clone: HB15e) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Raji-Zellen
• Erfolgreiche Detektion in FC: Human monocyte-derived mature dendritic cells

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65581-1-MR bindet in IF/ICC, FC CD83 und zeigt Reaktivität mit human, nicht-humane Primaten

• Getestete Reaktivität: human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane CD83-transfizierte Cos-Zellen
• Vollständiger Name: CD83 molecule
• Berechnetes Molekulargewicht: 205 aa, 23 kDa
• GenBank-Zugangsnummer: BC030830
• Gene symbol: CD83
• Gene ID (NCBI): 9308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).