CD83 Rekombinanter Antikörper

CD83 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241002B7

Kat-Nr. : 98119-1-RR

Synonyme

241002B7, CD83 antigen, mCD83

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  FC experiment of mouse splenocytes using 98119-1-RR

  1x10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD83 Rabbit Recombinant Antibody (98119-1-RR, Clone: 241002B7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98119-1-RR

  1x10^6 LPS-treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD83 Rabbit Recombinant Antibody (98119-1-RR, Clone: 241002B7) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD83 Rabbit Recombinant Antibody (98119-1-RR, Clone: 241002B7) (black, dashed), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98119-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98119-1-RR against Mouse CD83 were performed. The affinity constant is 0.199 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98119-1-RR bindet in FC CD83 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD83 fusion protein Eg1387
• Vollständiger Name: CD83 antigen
• Berechnetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: NM_009856.3
• Gene symbol: Cd83
• Gene ID (NCBI): 12522
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).