CD83 Rekombinanter Antikörper

CD83 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

FC

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

HB15e

Kat-Nr. : CL750-65581

Synonyme

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

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  FC experiment of human monocyte-derived mature dendritic cells using CL750-65581

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD83 (HB15e) Mouse IgG2a Recombinant Antibody (CL750-65581, Clone: HB15e) (red) or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human monocyte-derived mature dendritic cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL750-65581 bindet in FC CD83 und zeigt Reaktivität mit human, nicht-humane Primaten

• Getestete Reaktivität: human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane CD83-transfizierte Cos-Zellen
• Vollständiger Name: CD83 molecule
• Berechnetes Molekulargewicht: 205 aa, 23 kDa
• GenBank-Zugangsnummer: BC030830
• Gene symbol: CD83
• Gene ID (NCBI): 9308
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).