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CD84 Rekombinanter Antikörper

CD84 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241342F3

Kat-Nr. : 84245-2-PBS

Synonyme

241342F3, CD84 molecule, Cell surface antigen MAX.3, hCD84, Hly9 beta

Filter:
  • Western Blot (WB) analysis of various lysates using CD84 Recombinant antibody (84245-2-RR)

    WB analysis using 84245-2-RR (same clone as 84245-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84245-2-RR (CD84 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84245-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD84 Recombinant antibody (84245-2-RR)

    IF Staining of Raji using 84245-2-RR (same clone as 84245-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD84 antibody (84245-2-RR, Clone: 241342F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84245-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84245-2-RR

    Affinity and Kinetic Characterization of 84245-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84245-2-RR against Human CD84 were performed. The affinity constant is 0.409 nM.

Geprüfte Anwendungen

Produktinformation

84245-2-PBS bindet in WB, IF/ICC, Indirect ELISA CD84 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name CD84 molecule
Berechnetes Molekulargewicht 345 aa, 39 kDa
Beobachtetes Molekulargewicht70 kDa
GenBank-ZugangsnummerBC020063
Gene symbol CD84
Gene ID (NCBI) 8832
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).