CD84 Rekombinanter Antikörper

CD84 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241342F3

Kat-Nr. : 84245-2-RR

Synonyme

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

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  WB analysis using 84245-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84245-2-RR (CD84 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of Raji using 84245-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD84 antibody (84245-2-RR, Clone: 241342F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84245-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84245-2-RR against Human CD84 were performed. The affinity constant is 0.409 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, Ramos-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84245-2-RR bindet in WB, IF/ICC, ELISA CD84 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD84 molecule
• Berechnetes Molekulargewicht: 345 aa, 39 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC020063
• Gene symbol: CD84
• Gene ID (NCBI): 8832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).