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CD86 Polyklonaler Antikörper

CD86 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30691-1-PBS

Synonyme

B7, B7 2, B7.2, B70, B7-2

Filter:
  • Western Blot (WB) analysis of various lysates using CD86 Polyclonal antibody (30691-1-AP)

    WB analysis using 30691-1-AP (same clone as 30691-1-PBS)

    DC2.4 cells were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30691-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD86 Polyclonal antibody (30691-1-AP)

    WB analysis using 30691-1-AP (same clone as 30691-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30691-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30691-1-PBS bindet in WB, Indirect ELISA CD86 und zeigt Reaktivität mit Maus, Ratten

Getestete Reaktivität Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD86 fusion protein Ag33514
Vollständiger Name CD86 antigen
Berechnetes Molekulargewicht35 kDa
Beobachtetes Molekulargewicht60 kDa
GenBank-ZugangsnummerNM_019388
Gene symbol Cd86
Gene ID (NCBI) 12524
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)