CD86 Monoklonaler Antikörper

CD86 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Ratte

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

24F

Kat-Nr. : 65224-1-Ig

Synonyme

CD86 molecule, B7-2, B7 2, 24F

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• 

  FC experiment of Wistar Rat splenocytes using 65224-1-Ig

  1X10^6 unstimulated or LPS-stimulated Wistar rat splenocytes were surface stained with 0.5 ug Anti-Rat CD86 (65224-1-Ig, Clone:24F) and FITC anti-mouse IgG1 Antibody at dilution 1:100. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS-stimulated Wistar rat splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

65224-1-Ig bindet in IF, FC CD86 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
• Vollständiger Name: CD86 molecule
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: NM_020081
• Gene symbol: Cd86
• Gene ID (NCBI): 56822
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Publikationen

Species	Application	Title
rat	IF,FC	Prostaglandins Other Lipid Mediat; Protective role of Yougui Yin in experimental knee osteoarthritis: from the perspective of macrophage polarization; Authors - Zhongqing Wu