CD86 Monoklonaler Antikörper

CD86 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

APC Fluorescent Dye

Publikationen(6)

CloneNo.

BU63

Kat-Nr. : APC-65165

Synonyme

CD28LG2, BU63, B70, B7 2, Activation B7-2 antigen



Geprüfte Anwendungen

Erfolgreiche Detektion in FChumane PBMCs

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 2 publications below
FCSee 4 publications below

Produktinformation

APC-65165 bindet in IF, FC CD86 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen B-lymphoblastoide Zelllinie ARH 77
Vollständiger Name CD86 molecule
Berechnetes Molekulargewicht 329 aa, 38 kDa
GenBank-ZugangsnummerBC040261
Gene symbol CD86
Gene ID (NCBI) 942
Konjugation APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Reinigungsmethode Der gereinigte Antikörper ist mit Allophycocyanin (APC) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
Lagerungspuffer PBS with 0.09% sodium azide
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
FC protocol for APC CD86 antibody APC-65165Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanFC

Cell Death Discov

A positive feedback loop: RAD18-YAP-TGF-β between triple-negative breast cancer and macrophages regulates cancer stemness and progression.

Authors - Xueqi Yan
humanFC

Inflammation

ROS-Induced Gingival Fibroblast Senescence: Implications in Exacerbating Inflammatory Responses in Periodontal Disease

Authors - Shuling Guo
humanFC

Front Immunol

Identification of a distinct cluster of GDF15high macrophages induced by in vitro differentiation exhibiting anti-inflammatory activities

Authors - Chaochao Dai
humanFC

J Agric Food Chem

Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags

Authors - Zilong Zhao
humanIF

Adv Sci (Weinh)

Senescent Fibroblasts Drive FAP/OLN Imbalance Through mTOR Signaling to Exacerbate Inflammation and Bone Resorption in Periodontitis

Authors - Chenghu Yin
humanIF

Cell Death Dis

Tumor-derived exosomal CCT6A serves as a matchmaker introducing chemokines to tumor-associated macrophages in pancreatic ductal adenocarcinoma

Authors - Tianyin Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Julie (Verified Customer) (08-26-2025)

Used at 5uL per 100uL sample containing approximately 4-5 x 10^5 cells, has worked well every time. Used as a single stain and samples ran within one hour without fixing.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: NA
  • Cell Tissue Type: THP-1