CD86 Rekombinanter Antikörper

CD86 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

BU63

Kat-Nr. : CL647-65514

Synonyme

Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70, BU63

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  FC experiment of human PBMCs using CL647-65514

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD86 Rabbit Recombinant Antibody (CL647-65514, Clone:BU63) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65514

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD86 Rabbit Recombinant Antibody (CL647-65514, Clone:BU63) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65514

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD86 Rabbit Recombinant Antibody (CL647-65514, Clone:BU63) or 5 ul CoraLite® Plus 647 Anti-Human CD86 Mouse Monoclonal Antibody (CL647-65165, Clone:BU63). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65514 bindet in FC CD86 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: B-lymphoblastoide Zelllinie ARH 77
• Vollständiger Name: CD86 molecule
• Berechnetes Molekulargewicht: 329 aa, 38 kDa
• GenBank-Zugangsnummer: BC040261
• Gene symbol: CD86
• Gene ID (NCBI): 942
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)