CD86 Rekombinanter Antikörper

CD86 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230476B8

Kat-Nr. : CL647-98025

Synonyme

B7-2, B70, B7.2, B7 2, B7

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  FC experiment of mouse splenocytes using CL647-98025

  1x10^6 unstimulated (left) or LPS treated (right) mouse splenocytes were surface stained with 0.25ug CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL647-98025, Clone: 230476B8). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL647-98025

  1x10^6 LPS treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL647-98025, Clone: 230476B8) (red) or Isotype Control (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL647-98025, Clone: 230476B8) (black). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98025 bindet in FC CD86 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD86 antigen
• Berechnetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_019388
• Gene symbol: Cd86
• Gene ID (NCBI): 12524
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)