CD86 Rekombinanter Antikörper

CD86 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

BU63

Kat-Nr. : FITC-65592

Synonyme

CD28LG2, BU63, B70, B7 2, Activation B7-2 antigen

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  FC experiment of human PBMCs using FITC-65592

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD14 Rabbit Recombinant Antibody (APC-98040, Clone: 230332D7), and 5 ul FITC Plus Anti-Human CD86 (BU63) Mouse IgG2a RecAb (FITC-65592, Clone: BU63) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-65592

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 (BU63) Mouse IgG2a RecAb (FITC-65592, Clone: BU63) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65592 bindet in FC CD86 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: B-lymphoblastoide Zelllinie ARH 77
• Vollständiger Name: CD86 molecule
• Berechnetes Molekulargewicht: 329 aa, 38 kDa
• GenBank-Zugangsnummer: BC040261
• Gene symbol: CD86
• Gene ID (NCBI): 942
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.