CD86 Rekombinanter Antikörper

CD86 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

GL1

Kat-Nr. : PE-FcA65673

Synonyme

B7, B7 2, B7.2, B70, B7-2

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  FC experiment of mouse splenocytes using PE-FcA65673

  1x10^6 untreated or LPS treated mouse splenocytes were surface stained with 0.1 ug PE Anti-Mouse CD86 (GL1) Rabbit IgG RecAb (PE-FcA65673, Clone: GL1). Cells were not fixed.

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  FC experiment of mouse splenocytes using PE-FcA65673

  1x10^6 LPS-treated mouse splenocytes were surface stained with 0.1 ug PE Anti-Mouse CD86 (GL1) Rabbit IgG RecAb (PE-FcA65673, Clone: GL1)(red) or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.1 ug PE Anti-Mouse CD86 (GL1) Rabbit IgG RecAb (PE-FcA65673, Clone: GL1) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-FcA65673 bindet in FC CD86 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD86 antigen
• GenBank-Zugangsnummer: BC013807
• Gene symbol: Cd86
• Gene ID (NCBI): 12524
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)