CD86 Multi-Recombinanter Antikörper

CD86 Multi-Recombinant Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : RMX00027

Synonyme

Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70, BU63

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  WB analysis using RMX00027

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00027 (CD86 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using RMX00027

  IP result of anti-CD86 (IP:RMX00027, 4ug; Detection:RMX00027 1:8000) with Raji cells lysate 2200 ug.

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  IF Staining of Raji using RMX00027

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD86 antibody (RMX00027, Clone: 240429A6-H1L1,240429G11-H1L1,240429G12-H1L1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, Ramos-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

RMX00027 bindet in WB, IF/ICC, IP, ELISA CD86 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Multi-Recombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD86 molecule
• Berechnetes Molekulargewicht: 329 aa, 38 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_175862
• Gene symbol: CD86
• Gene ID (NCBI): 942
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.