CD86 (C-terminal) Polyklonaler Antikörper

CD86 (C-terminal) Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26903-1-PBS

Synonyme

CD86, Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70

---

• 

  WB analysis using 26903-1-AP (same clone as 26903-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26903-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 26903-1-AP (same clone as 26903-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26903-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 26903-1-AP (same clone as 26903-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26903-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

26903-1-PBS bindet in WB, IHC, Indirect ELISA CD86 (C-terminal) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD86 (C-terminal) fusion protein Ag25432
• Vollständiger Name: CD86 molecule
• Berechnetes Molekulargewicht: 329 aa, 38 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC040261
• Gene symbol: CD86
• Gene ID (NCBI): 942
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.