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CD8a Rekombinanter Antikörper

CD8a Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242879G4

Kat-Nr. : 85977-3-PBS

Synonyme

CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35

Filter:
  • Western Blot (WB) analysis of mouse thymus tissue using CD8a Recombinant antibody (85977-3-RR)

    WB analysis of mouse thymus using 85977-3-RR (same clone as 85977-3-PBS)

    Mouse thymus tissue was subjected to SDS PAGE followed by western blot with 85977-3-RR (CD8A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85977-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85977-3-PBS bindet in WB, Indirect ELISA CD8a und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD8a fusion protein Eg3265
Vollständiger Name CD8 antigen, alpha chain
Berechnetes Molekulargewicht27 kDa
Beobachtetes Molekulargewicht32-35 kDa
GenBank-ZugangsnummerNM_001081110.2
Gene symbol Cd8a
Gene ID (NCBI) 12525
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 is composed of two disulfide-linked chains and can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).