CD8a Rekombinanter Antikörper

CD8a Rekombinant Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

243018F10

Kat-Nr. : 86063-2-RR

Synonyme

CD8, CD8a molecule, Leu2, MAL, p32

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  IHC staining of human tonsillitis using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocellular cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocellular cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 86063-2-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86063-2-RR (CD8a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 86063-2-RR

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (86063-2-RR, Clone: 243018F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of human tonsillitis using 86063-2-RR

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD8a antibody (86063-2-RR, Clone: 243018F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86063-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86063-2-RR against Human CD8a were performed. The affinity constant is 13.2 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86063-2-RR bindet in IHC, IF/ICC, ELISA CD8a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD8a fusion protein Eg0819
• Vollständiger Name: CD8a molecule
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: NM_001145873.1
• Gene symbol: CD8A
• Gene ID (NCBI): 925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 22-182 aa of human CD8a, the alpha chain of CD8 molecule.