CD8a Rekombinanter Antikörper

CD8a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242879C10

Kat-Nr. : 98472-2-PBS

Synonyme

CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35

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  FC experiment of mouse splenocytes using 98472-2-RR (same clone as 98472-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8a Rabbit RecAb (98472-2-RR, Clone: 242879C10) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98472-2-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98472-2-RR (same clone as 98472-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8a Rabbit RecAb (98472-2-RR, Clone: 242879C10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98472-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98472-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98472-2-RR against Mouse CD8a were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

98472-2-PBS bindet in FC CD8a und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD8a fusion protein Eg3265
• Vollständiger Name: CD8 antigen, alpha chain
• Berechnetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: NM_001081110.2
• Gene symbol: Cd8a
• Gene ID (NCBI): 12525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).