CD8a Rekombinanter Antikörper

CD8a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

OKT8

Rezensionen (1)

Kat-Nr. : APC-FcA65506

Synonyme

CD8, p32, OKT8, MAL, Leu2

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  FC experiment of human PBMCs using APC-FcA65506

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD4 and 5 ul PE Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Antibody (PE-FcA65506, Clone: OKT8) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-FcA65506 bindet in FC CD8a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane Thymozyten
• Vollständiger Name: CD8a molecule
• Berechnetes Molekulargewicht: 235 aa, 26 kDa
• GenBank-Zugangsnummer: BC025715
• Gene symbol: CD8A
• Gene ID (NCBI): 925
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Eduardo (Verified Customer) (07-02-2025)

The titration results for the anti-CD8 antibody conjugated to APC show excellent performance across a range of concentrations. Notably, the signal intensity increases proportionally with antibody concentration, with clear separation between positive and negative populations beginning as early as the 0.25:150 dilution. Optimal staining appears to be achieved at the 2:150 and 4:150 dilutions, with 4:150 offering improved resolution between CD8^dim and CD8^bright populations due to enhanced signal separation.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 4:150 , 2:150 , 1:150, 0.5:150, 0.25:150
• Cell Tissue Type: Human PBMCs

APC CD8a Antibody Flow Cytometry validation (4:150 , 2:150 , 1:150, 0.5:150, 0.25:150 dilution) in Human PBMCs (Cat no:APC-FcA65506)