CD8b Rekombinanter Antikörper

CD8b Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250480F2

Kat-Nr. : 86024-1-RR

Synonyme

Cd8b1, Ly-3, Lymphocyte antigen 3, Lyt3, Lyt-3

---

• 

  WB analysis using 86024-1-RR

  Mouse thymus tissue was subjected to SDS PAGE followed by western blot with 86024-1-RR (CD8 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86024-1-RR

  Mouse spleen tissue was subjected to SDS PAGE followed by western blot with 86024-1-RR (CD8 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86024-1-RR bindet in WB, ELISA CD8b und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD8b fusion protein Eg4275
• Vollständiger Name: CD8 antigen, beta chain 1
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: NM_009858.2
• Gene symbol: Cd8b1
• Gene ID (NCBI): 12526
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.