CD8b Rekombinanter Antikörper

CD8b Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241326A7

Kat-Nr. : 98103-1-RR

Synonyme

CD8, 241326A7, Ly3, Leu2, CD8B1

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  FC experiment of human PBMCs using 98103-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8b Rabbit Recombinant Antibody (98103-1-RR, Clone: 241326A7) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98103-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8b Rabbit Recombinant Antibody (98103-1-RR, Clone: 241326A7)(red), or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98103-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98103-1-RR against Human CD8b were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98103-1-RR bindet in FC CD8b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD8b molecule
• Berechnetes Molekulargewicht: 243 aa, 27 kDa
• GenBank-Zugangsnummer: BC100913
• Gene symbol: CD8B
• Gene ID (NCBI): 926
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on the surface of cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.