CD8b Rekombinanter Antikörper

CD8b Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250480F2

Kat-Nr. : 98461-3-PBS

Synonyme

Cd8b1, Ly-3, Lymphocyte antigen 3, Lyt3, Lyt-3

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  FC experiment of mouse splenocytes using 98461-3-RR (same clone as 98461-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8b Rabbit RecAb (98461-3-RR, Clone: 250480F2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 98461-3-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98461-3-RR (same clone as 98461-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8b Rabbit RecAb (98461-3-RR, Clone: 250480F2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98461-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98461-3-PBS bindet in FC CD8b und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD8b fusion protein Eg4275
• Vollständiger Name: CD8 antigen, beta chain 1
• Berechnetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_009858.2
• Gene symbol: Cd8b1
• Gene ID (NCBI): 12526
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on the surface of cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.