CD8b Rekombinanter Antikörper

CD8b Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250480F2

Kat-Nr. : 98461-3-RR

Synonyme

Cd8b1, Ly-3, Lymphocyte antigen 3, Lyt3, Lyt-3

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  FC experiment of mouse splenocytes using 98461-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8b Rabbit RecAb (98461-3-RR, Clone: 250480F2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98461-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD8b Rabbit RecAb (98461-3-RR, Clone: 250480F2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98461-3-RR bindet in FC CD8b und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD8b fusion protein Eg4275
• Vollständiger Name: CD8 antigen, beta chain 1
• Berechnetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_009858.2
• Gene symbol: Cd8b1
• Gene ID (NCBI): 12526
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on the surface of cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.