CD9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (9)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(341)

Kat-Nr. : 20597-1-AP

Synonyme

5H9, 5H9 antigen, BA2, BTCC 1, CD9 antigen

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  WB analysis using 20597-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20597-1-AP (CD9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 20597-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 20597-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (20597-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HeLa-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 325 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

20597-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CD9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Huhn, Kaninchen, Maus, Ratte, Zebrafisch, Ziege, Fledermaus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD9 fusion protein Ag14546
• Vollständiger Name: CD9 molecule
• Berechnetes Molekulargewicht: 228 aa, 25 kDa
• Beobachtetes Molekulargewicht: 23-30 kDa
• GenBank-Zugangsnummer: BC011988
• Gene symbol: CD9
• Gene ID (NCBI): 928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).

Publikationen

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis; Authors - Chuan Yang
human	WB	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
human	WB	Cell Res; In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.; Authors - Zheng Fu
	WB	Bioact Mater; Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis; Authors - Wei Zhang
rat	WB,IF	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang