CD9 Rekombinanter Antikörper

CD9 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242432B5

Kat-Nr. : 84801-13-PBS

Synonyme

CD9 antigen, MIC3, P24, Tspan 29, TSPAN29

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  WB analysis using 84801-13-RR (same clone as 84801-13-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84801-13-RR (CD9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84801-13-RR (same clone as 84801-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84801-13-RR (same clone as 84801-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84801-13-RR (same clone as 84801-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84801-13-RR (same clone as 84801-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84801-13-RR (same clone as 84801-13-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84801-13-RR (same clone as 84801-13-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 84801-13-RR (same clone as 84801-13-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 84801-13-RR (same clone as 84801-13-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84801-13-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 84801-13-RR (same clone as 84801-13-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD9 antibody (84801-13-RR, Clone: 242432B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-13-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84801-13-PBS bindet in WB, IHC, IF-P, Indirect ELISA CD9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD9 fusion protein Eg1397
• Vollständiger Name: CD9 antigen
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: NM_007657.3
• Gene symbol: Cd9
• Gene ID (NCBI): 12527
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and supports myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in the fertilization of mammals (PMID: 25536312).