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CD9 Rekombinanter Antikörper

CD9 Rekombinant Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242432C2

Kat-Nr. : 84801-4-PBS

Synonyme

242432C2, CD9 antigen, MIC3, P24, Tspan 29

Filter:
  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD9 Recombinant antibody (84801-4-RR)

    IHC staining of mouse kidney using 84801-4-RR (same clone as 84801-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-4-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD9 Recombinant antibody (84801-4-RR)

    IHC staining of mouse kidney using 84801-4-RR (same clone as 84801-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84801-4-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84801-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD9 Recombinant antibody (84801-4-RR)

    IF Staining of RAW 264.7 using 84801-4-RR (same clone as 84801-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD9 antibody (84801-4-RR, Clone: 242432C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84801-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84801-4-RR

    Affinity and Kinetic Characterization of 84801-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84801-4-RR against Mouse CD9 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84801-4-PBS bindet in IHC, IF/ICC, Indirect ELISA CD9 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD9 fusion protein Eg1397
Vollständiger Name CD9 antigen
Berechnetes Molekulargewicht25 kDa
GenBank-ZugangsnummerNM_007657.3
Gene symbol Cd9
Gene ID (NCBI) 12527
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and supports myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in the fertilization of mammals (PMID: 25536312).