CD9 Rekombinanter Antikörper

CD9 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240830E6

Kat-Nr. : 98095-1-RR

Synonyme

240830E6, 5H9, 5H9 antigen, BA2, BTCC 1

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  FC experiment of human PBMCs using 98095-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone:240830E6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Human CD3 (OKT3) . Cells were not fixed.

• 

  FC experiment of human PBMCs using 98095-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone:240830E6)(red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98095-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone:240830E6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed.

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  FC experiment of human peripheral blood platelets using 98095-1-RR

  1x10^6 human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone: 240830E6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98095-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98095-1-RR against Human CD9 were performed. The affinity constant is 407 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs, human peripheral blood platelets

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98095-1-RR bindet in FC CD9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD9 fusion protein Eg1037
• Vollständiger Name: CD9 molecule
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_001769.4
• Gene symbol: CD9
• Gene ID (NCBI): 928
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405).