CD90 Monoklonaler Antikörper

CD90 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D7D11

Kat-Nr. : 66766-1-PBS

Synonyme

CD90 / Thy1, THY1, 2D7D11, Thy 1 antigen, Thy 1 cell surface antigen

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  WB analysis using 66766-1-Ig (same clone as 66766-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 66766-1-Ig (same clone as 66766-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 66766-1-Ig (same clone as 66766-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66766-1-Ig (same clone as 66766-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66766-1-Ig (same clone as 66766-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 66766-1-Ig (same clone as 66766-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66766-1-Ig (same clone as 66766-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66766-1-Ig (same clone as 66766-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66766-1-Ig (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66766-1-Ig (CD90 antibody) at dilution of 1:3900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66766-1-Ig (same clone as 66766-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66766-1-Ig (CD90 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66766-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66766-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA CD90 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD90 fusion protein Ag25603
• Vollständiger Name: Thy-1 cell surface antigen
• Berechnetes Molekulargewicht: 161 aa, 18 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC065559
• Gene symbol: CD90/Thy1
• Gene ID (NCBI): 7070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.