CD90 Rekombinanter Antikörper

CD90 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241290B1

Kat-Nr. : 84180-1-PBS

Synonyme

CD90 / Thy1, THY1, 241290B1, Thy 1 antigen, Thy 1 cell surface antigen

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  WB analysis using 84180-1-RR (same clone as 84180-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84180-1-RR (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84180-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 84180-1-RR (same clone as 84180-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed SH-SY5Y cells using CD90 antibody (84180-1-RR, Clone: 241290B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84180-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01072-1

  Cytometric bead array standard curve of MP01072-1, CD90 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84180-1-PBS. Detection antibody: 84180-2-PBS. Standard: RP02312. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 84180-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84180-1-RR against Human CD90 were performed. The affinity constant is 0.383 nM.

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Geprüfte Anwendungen

Produktinformation

84180-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA CD90 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Thy-1 cell surface antigen
• Berechnetes Molekulargewicht: 161 aa, 18 kDa
• Beobachtetes Molekulargewicht: 25-35 kDa
• GenBank-Zugangsnummer: BC065559
• Gene symbol: CD90/Thy1
• Gene ID (NCBI): 7070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD90, also known as THY1, is a 25-35 kDa protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.