CD90 Rekombinanter Antikörper

CD90 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241290B1

Kat-Nr. : 84180-1-RR

Synonyme

CD90 / Thy1, THY1, 241290B1, Thy 1 antigen, Thy 1 cell surface antigen

---

• 

  WB analysis using 84180-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84180-1-RR (CD90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SH-SY5Y using 84180-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed SH-SY5Y cells using CD90 antibody (84180-1-RR, Clone: 241290B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84180-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84180-1-RR against Human CD90 were performed. The affinity constant is 0.383 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, fetales humanes Hirngewebe, Maushirngewebe, PC-12-Zellen, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84180-1-RR bindet in WB, IF/ICC, ELISA CD90 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Thy-1 cell surface antigen
• Berechnetes Molekulargewicht: 161 aa, 18 kDa
• Beobachtetes Molekulargewicht: 25-35 kDa
• GenBank-Zugangsnummer: BC065559
• Gene symbol: CD90/Thy1
• Gene ID (NCBI): 7070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD90, also known as THY1, is a 25-35 kDa protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.