CD90 Polyklonaler Antikörper

CD90 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 27178-1-AP

Synonyme

CD90 / Thy1, THY1, Thy 1 antigen, Thy 1 cell surface antigen, Thy 1 membrane glycoprotein

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  WB analysis using 27178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27178-1-AP (CD90 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, Maushirngewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 10 publications below

Produktinformation

27178-1-AP bindet in WB, IHC, IF, ELISA CD90 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD90 fusion protein Ag25603
• Vollständiger Name: Thy-1 cell surface antigen
• Berechnetes Molekulargewicht: 161 aa, 18 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC065559
• Gene symbol: CD90/Thy1
• Gene ID (NCBI): 7070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

Publikationen

Species	Application	Title
rat	IF	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
mouse	IF	J Orthop Translat; Osteostaticytes: A novel osteoclast subset couples bone resorption and bone formation; Authors - Zhiyuan Wei
human	IF	Stem Cells; Nicotinamide riboside modulates HIF-1 signaling to maintain and enhance odontoblastic differentiation in human dental pulp stem cells; Authors - Peimeng Zhan
human	IF	Transl Oncol; A machine learning-based prognostic signature utilizing MSC proteomics for predicting bladder cancer prognosis and treatment response; Authors - Xinyu Zhang
rat	IF	Neurogastroenterol Motil; Construction and identification of an immortalized rat intestinal smooth muscle cell line.; Authors - Huiling Chen
rat	IF	ACS Nano; Micronano Titanium Accelerates Mesenchymal Stem Cells Aging through the Activation of Senescence-Associated Secretory Phenotype; Authors - Xuan Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christelle (Verified Customer) (01-06-2025)

Immunofluorescent analysis of (PFA 4%) fixed human renal mesangial cells using CD90 antibody (27178-1-AP) at dilution of 1:50 and AF488-Conjugated Donkey Anti-Rabbit IgG (Life Technologies), Star Red membrane probe (Abberior, yellow) and DAPI (blue). 2 files uploaded : 1 aquisition without the membrane probe and the same aquisition with the membrane probe.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human Renal Mesangial Cells (HRMC, primary cells)

CD90 Antibody Immunofluorescence validation (1:50 dilution) in Human Renal Mesangial Cells (HRMC, primary cells) (Cat no:27178-1-AP)