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CD90 Rekombinanter Antikörper

CD90 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241388E2

Kat-Nr. : 84206-3-PBS

Synonyme

241388E2, Thy1, Thy-1, Thy-1 antigen, Thy-1 membrane glycoprotein

Filter:
  • Western Blot (WB) analysis of various lysates using CD90 Recombinant antibody (84206-3-RR)

    WB analysis using 84206-3-RR (same clone as 84206-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84206-3-RR (CD90/Thy1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84206-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CD90 Recombinant antibody (84206-3-RR)

    IF Staining of PC-12 using 84206-3-RR (same clone as 84206-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD90/Thy1 antibody (84206-3-RR, Clone: 241388E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84206-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01132-1

    Cytometric bead array standard curve of MP01132-1

    Cytometric bead array standard curve of MP01132-1, RAT CD90/Thy1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84206-3-PBS. Detection antibody: 84206-1-PBS. Standard: Eg1463. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP01132-2

    Cytometric bead array standard curve of MP01132-2

    Cytometric bead array standard curve of MP01132-2, RAT CD90/Thy1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84206-3-PBS. Detection antibody: 84206-2-PBS. Standard: Eg1463. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84206-3-RR

    Affinity and Kinetic Characterization of 84206-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84206-3-RR against Rat CD90/Thy1 were performed. The affinity constant is 27.1 pM.

Geprüfte Anwendungen

Produktinformation

84206-3-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA CD90 und zeigt Reaktivität mit Ratten

Getestete Reaktivität Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD90 fusion protein Eg1463
Vollständiger Name Thy-1 cell surface antigen
Berechnetes Molekulargewicht18 kDa
Beobachtetes Molekulargewicht25 kDa
GenBank-ZugangsnummerNM_012673.2
Gene symbol Thy1
Gene ID (NCBI) 24832
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.