CD90 Rekombinanter Antikörper

CD90 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241388E2

Kat-Nr. : 84206-3-RR

Synonyme

241388E2, Thy1, Thy-1, Thy-1 antigen, Thy-1 membrane glycoprotein

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  WB analysis using 84206-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84206-3-RR (CD90/Thy1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using 84206-3-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD90/Thy1 antibody (84206-3-RR, Clone: 241388E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84206-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84206-3-RR against Rat CD90/Thy1 were performed. The affinity constant is 27.1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84206-3-RR bindet in WB, IF/ICC, ELISA CD90 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD90 fusion protein Eg1463
• Vollständiger Name: Thy-1 cell surface antigen
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_012673.2
• Gene symbol: Thy1
• Gene ID (NCBI): 24832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.