CD93 Polyklonaler Antikörper

CD93 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33313-1-AP

Synonyme

Aa4, C1q/MBL/SPA receptor, C1qR(p), C1qr1, C1qRp

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  WB analysis using 33313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33313-1-AP (CD93 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 33313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33313-1-AP (CD93 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 33313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33313-1-AP (CD93 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 33313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33313-1-AP (CD93 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bEnd.3-Zellen, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

33313-1-AP bindet in WB, IHC, ELISA CD93 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD93 fusion protein Eg3116
• Vollständiger Name: CD93 antigen
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 90 kDa
• GenBank-Zugangsnummer: NM_010740.3
• Gene symbol: Cd93
• Gene ID (NCBI): 17064
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD93, also known as C1qR or C1qR(p), is a single-pass type I transmembrane glycoprotein and a member of the group 14 of the C-type lectin-like domain (CTLD) superfamily, which also includes endosialin/CD248, thrombomodulin, and CLEC14A (PMID: 31287944). CD93 consists of a C-type lectin-like domain, five EGF-like repeats, a mucin-like domain, a transmembrane domain and a cytoplasmic domain (PMID: 28912033). CD93 is mainly expressed on endothelial cells and has been reported to be expressed by neurons and various cells of the hematopoietic system, including monocytes, neutrophils, B cells, natural killer cells, platelets and hematopoietic stem cells (PMID: 37443812). CD93 plays a role in various physiological processes including angiogenesis, inflammation, and cell adhesion (PMID: 31287944).