CD97 Polyklonaler Antikörper

CD97 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 13071-1-AP

Synonyme

CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

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Galerie der Validierungsdaten

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  WB analysis using 13071-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13071-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 13071-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13071-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 13071-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13071-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 13071-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13071-1-AP (CD97 antibody) at dilution of 1:100 (under 10x lens).

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  FC experiment of K-562 using 13071-1-AP

  1X10^6 K-562 cells were stained with 0.2ug CD97 antibody (13071-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13071-1-AP bindet in WB, IHC, FC, ELISA CD97 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD97 fusion protein Ag3745
• Vollständiger Name: CD97 molecule
• Berechnetes Molekulargewicht: 722 aa, 79 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC026690
• Gene symbol: CD97
• Gene ID (NCBI): 976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fiona (Verified Customer) (01-09-2020)

Used sample to screen cell lines for expression of the CD97 protein. Didn't observe band where expected for U-937 cells (positive control) however assay requires further optimisation. Otherwise blot was clean. Specific clean staining observed when used for immunofluorescence.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Multiple cell lines