CD97 Rekombinanter Antikörper

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• KD/KO Validated

CD97 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241335A11

Kat-Nr. : 84255-6-PBS

Synonyme

241335A11, ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta

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  WB analysis using 84255-6-RR (same clone as 84255-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84255-6-RR (CD97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 84255-6-RR (same clone as 84255-6-PBS)

  WB result of CD97 antibody (84255-6-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD97 transfected HepG2 cells. This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84255-6-RR (same clone as 84255-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84255-6-RR (CD97 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 84255-6-RR (same clone as 84255-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (84255-6-RR, Clone: 241335A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84255-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84255-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84255-6-RR against Human CD97 were performed. The affinity constant is 2.73 nM.

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Geprüfte Anwendungen

Produktinformation

84255-6-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD97 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD97 molecule
• Berechnetes Molekulargewicht: 722 aa, 79 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC026690
• Gene symbol: CD97
• Gene ID (NCBI): 976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).