CD97 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

CD97 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241335A11

Kat-Nr. : 84255-6-RR

Synonyme

241335A11, ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta

---

• 

  WB analysis using 84255-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84255-6-RR (CD97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 84255-6-RR

  WB result of CD97 antibody (84255-6-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD97 transfected HepG2 cells.

• 

  IHC staining of human tonsillitis using 84255-6-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84255-6-RR (CD97 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Jurkat using 84255-6-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (84255-6-RR, Clone: 241335A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84255-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84255-6-RR against Human CD97 were performed. The affinity constant is 2.73 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, BGC-823-Zellen, DU 145-Zellen, HepG2-Zellen, HL-60-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84255-6-RR bindet in WB, IHC, IF/ICC, ELISA CD97 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD97 molecule
• Berechnetes Molekulargewicht: 722 aa, 79 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC026690
• Gene symbol: CD97
• Gene ID (NCBI): 976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).