CD98/SLC3A2 Rekombinanter Antikörper

CD98/SLC3A2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241247F12

Kat-Nr. : 84040-7-RR

Synonyme

4F2 cell-surface antigen heavy chain, 4F2hc, Amino acid transporter heavy chain SLC3A2, CD98, Mdu1

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  WB analysis of mouse kidney using 84040-7-RR

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 84040-7-RR

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 84040-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 84040-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84040-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 84040-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84040-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84040-7-RR against Mouse CD98/SLC3A2 were performed. The affinity constant is 1.36 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84040-7-RR bindet in WB, IHC, ELISA CD98/SLC3A2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD98/SLC3A2 fusion protein Eg1374
• Vollständiger Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Berechnetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_008577.4
• Gene symbol: CD98/SLC3A2
• Gene ID (NCBI): 17254
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).