CD98/SLC3A2 Polyklonaler Antikörper

CD98/SLC3A2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32010-1-AP

Synonyme

CD98, SLC3A2, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2, MDU1

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  WB analysis using 32010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32010-1-AP (CD98/SLC3A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 32010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32010-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 32010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32010-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 32010-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CD98/SLC3A2 antibody (32010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MCF-7-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32010-1-AP bindet in WB, IHC, IF/ICC, ELISA CD98/SLC3A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD98/SLC3A2 fusion protein Eg1948
• Vollständiger Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 85-94 kDa, 120-130 kDa
• GenBank-Zugangsnummer: NM_002394.5
• Gene symbol: CD98
• Gene ID (NCBI): 6520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).