CD99 Polyklonaler Antikörper

CD99 Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 23079-1-AP

Synonyme

12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2

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Galerie der Validierungsdaten

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  WB analysis using 23079-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23079-1-AP (CD99 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 23079-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 23079-1-AP (CD99 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 23079-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 23079-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 23079-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 23079-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23079-1-AP (CD99 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Jurkat using 23079-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug CD99 antibody (23079-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, THP-1-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

23079-1-AP bindet in WB, IHC, IF, FC, ELISA CD99 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD99 fusion protein Ag19461
• Vollständiger Name: CD99 molecule
• Berechnetes Molekulargewicht: 19 kDa, 16 kDa, 17 kDa
• Beobachtetes Molekulargewicht: 28 kDa, 32 kDa
• GenBank-Zugangsnummer: BC021620
• Gene symbol: CD99
• Gene ID (NCBI): 4267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).

Publikationen

Species	Application	Title
pig	IHC,IF	J Anim Sci Biotechnol; Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis.; Authors - Lingkai Zhang
human	IHC	Oncol Lett; Metastatic extraskeletal Ewing's sarcoma treated with trabectedin: A case report.; Authors - Jorge Hernando-Cubero
human	IHC	Cell Death Dis; KDM5B promotes tumorigenesis of Ewing sarcoma via FBXW7/CCNE1 axis.; Authors - Binbin Chen