CD99 Monoklonaler Antikörper

CD99 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5B6A4

Kat-Nr. : 60354-1-PBS

Synonyme

12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2

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  WB analysis of human spleen using 60354-1-Ig (same clone as 60354-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60354-1-Ig (CD99 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60354-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60354-1-Ig (same clone as 60354-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60354-1-Ig (CD99 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60354-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60354-1-PBS bindet in WB, Indirect ELISA CD99 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD99 fusion protein Ag19474
• Vollständiger Name: CD99 molecule
• Berechnetes Molekulargewicht: 19 kDa, 16 kDa, 17 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC021620
• Gene symbol: CD99
• Gene ID (NCBI): 4267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing’s sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).