CD99 Rekombinanter Antikörper

CD99 Rekombinant Antikörper für FC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241737H1

Kat-Nr. : 98174-1-PBS

Synonyme

12E7, 241737H1, CD99 antigen, CD99 molecule, E2 antigen

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  FC experiment of human PBMCs using 98174-1-RR (same clone as 98174-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98174-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98174-1-RR (same clone as 98174-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98174-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98174-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98174-1-RR against Human CD99 were performed. The affinity constant is 3.61 nM.

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Geprüfte Anwendungen

Produktinformation

98174-1-PBS bindet in FC, Indirect ELISA CD99 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD99 molecule
• Berechnetes Molekulargewicht: 19 kDa, 16 kDa, 17 kDa
• GenBank-Zugangsnummer: BC021620
• Gene symbol: CD99
• Gene ID (NCBI): 4267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).