CD99 Rekombinanter Antikörper

CD99 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

241737H1

Kat-Nr. : CL594-98174

Synonyme

12E7, 241737H1, CD99 antigen, CD99 molecule, E2 antigen

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  FC experiment of human PBMCs using CL594-98174

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD99 Rabbit RecAb (CL594-98174, Clone: 241737H1) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL594-98174

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD99 Rabbit RecAb (CL594-98174, Clone:241737H1) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98174 bindet in FC CD99 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD99 molecule
• Berechnetes Molekulargewicht: 19 kDa, 16 kDa, 17 kDa
• GenBank-Zugangsnummer: BC021620
• Gene symbol: CD99
• Gene ID (NCBI): 4267
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).