CDA1 Polyklonaler Antikörper

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• KD/KO Validated

CDA1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12087-2-PBS

Synonyme

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  WB analysis of HeLa using 12087-2-AP (same clone as 12087-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12087-2-AP (same clone as 12087-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12087-2-AP (same clone as 12087-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 12087-2-AP (same clone as 12087-2-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 12087-2-AP (same clone as 12087-2-PBS)

  IP result of anti-CDA1 (IP:12087-2-AP, 4ug; Detection:12087-2-AP 1:1000) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12087-2-AP (same clone as 12087-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12087-2-AP (CDA1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12087-2-AP (same clone as 12087-2-PBS)

  Immunofluorescent analysis of Hela cells, using TSPYL2 antibody 12087-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12087-2-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CDA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDA1 fusion protein Ag2722
• Vollständiger Name: TSPY-like 2
• Berechnetes Molekulargewicht: 693 aa, 79 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC024270
• Gene symbol: CDA1
• Gene ID (NCBI): 64061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSPYL2 (also known as CINAP, CDA1, TSPX or DENTT) is a new member of the nucleosome assembly protein superfamily. TSPYL2 binds histones and facilitates nucleosome assembly. TSPYL2 is expressed in various tissues, highly in the pituitary gland and moderately in the adrenals, brain, testis, and ovary. Immunohistochemical staining analysis for TSPYL2 showed differential cytoplasmic and nuclear staining patterns in several cell types. Downregulated expression of TSPYL2 has been observed in several tumors, which suggests its role as a tumor suppressor. Although it is predicted that TSPYL2 has a molecular mass of 79.43 kDa, it is found that mammalian TSPYL2 appears at a size of 120 kDa by western blot analysis. The abundant acidic amino acid regions in TSPYL2 may cause its aberrant migration. In addition, the TSPYL2 protein is unstable and sensitive to proteasomal degradation.