CDC20 Rekombinanter Antikörper

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• KD/KO Validated

CDC20 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241925E12

Kat-Nr. : 84531-5-PBS

Synonyme

241925E12, CDC20A, p55CDC

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• 

  WB analysis using 84531-5-RR (same clone as 84531-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84531-5-RR (CDC20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 84531-5-RR (same clone as 84531-5-PBS)

  WB result of CDC20 antibody (84531-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC20 transfected HeLa cells. This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84531-5-RR (same clone as 84531-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84531-5-RR (CDC20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84531-5-RR (same clone as 84531-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84531-5-RR (CDC20 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84531-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84531-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84531-5-RR against Human CDC20 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84531-5-PBS bindet in WB, IHC, Indirect ELISA CDC20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDC20 fusion protein Ag0295
• Vollständiger Name: cell division cycle 20 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC001088
• Gene symbol: CDC20
• Gene ID (NCBI): 991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).