CDC25A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CDC25A Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55031-1-PBS

Synonyme

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• 

  WB analysis of HepG2 using 55031-1-AP (same clone as 55031-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 55031-1-AP (same clone as 55031-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 55031-1-AP (same clone as 55031-1-PBS)

  IP result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 55031-1-AP (same clone as 55031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 55031-1-AP (same clone as 55031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55031-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55031-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA CDC25A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cell division cycle 25 homolog A (S. pombe)
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_001789
• Gene symbol: CDC25A
• Gene ID (NCBI): 993
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.