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CDC25A Rekombinanter Antikörper

CDC25A Rekombinant Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240813F3

Kat-Nr. : 84145-2-PBS

Synonyme

M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3

Filter:
  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CDC25A Recombinant antibody (84145-2-RR)

    IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CDC25A Recombinant antibody (84145-2-RR)

    IHC staining of human stomach cancer using 84145-2-RR (same clone as 84145-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84145-2-RR (CDC25A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDC25A Recombinant antibody (84145-2-RR)

    IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDC25A Recombinant antibody (84145-2-RR)

    IF Staining of HepG2 using 84145-2-RR (same clone as 84145-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84145-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

84145-2-PBS bindet in IHC, IF/ICC, ELISA CDC25A und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name cell division cycle 25 homolog A (S. pombe)
Berechnetes Molekulargewicht 59 kDa
GenBank-ZugangsnummerNM_001789
Gene symbol CDC25A
Gene ID (NCBI) 993
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.