CDC25C Monoklonaler Antikörper

CDC25C Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B6B12

Kat-Nr. : 66912-1-PBS

Synonyme

2B6B12, CDC25, EC:3.1.3.48, M phase inducer phosphatase 3, M-phase inducer phosphatase 3

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  WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66912-1-PBS bindet in WB, Indirect ELISA CDC25C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CDC25C fusion protein Ag10240
• Vollständiger Name: cell division cycle 25 homolog C (S. pombe)
• Berechnetes Molekulargewicht: 473 aa, 53 kDa
• Beobachtetes Molekulargewicht: 46-53 kDa
• GenBank-Zugangsnummer: BC019089
• Gene symbol: CDC25C
• Gene ID (NCBI): 995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.