CDC27; APC3 Monoklonaler Antikörper

CDC27; APC3 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F10G2

Kat-Nr. : 67239-1-PBS

Synonyme

CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3

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  WB analysis of Jurkat using 67239-1-Ig (same clone as 67239-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67239-1-Ig (same clone as 67239-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67239-1-Ig (same clone as 67239-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 67239-1-Ig (same clone as 67239-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CDC27; APC3 (67239-1-Ig, Clone:2F10G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67239-1-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA CDC27; APC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CDC27; APC3 fusion protein Ag25588
• Vollständiger Name: cell division cycle 27 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC011656
• Gene symbol: CDC27
• Gene ID (NCBI): 996
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.