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CDC2L1 Polyklonaler Antikörper

CDC2L1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28613-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using CDC2L1 Polyclonal antibody (28613-1-AP)

    WB analysis using 28613-1-AP (same clone as 28613-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28613-1-AP (CDC2L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CDC2L1 Polyclonal antibody (28613-1-AP)

    WB analysis of HeLa using 28613-1-AP (same clone as 28613-1-PBS)

    WB result of CDC2L1 antibody (28613-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC2L1 transfected HeLa cells. This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human lung cancer using 28613-1-AP (same clone as 28613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human lung cancer using 28613-1-AP (same clone as 28613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human colon cancer using 28613-1-AP (same clone as 28613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human colon cancer using 28613-1-AP (same clone as 28613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDC2L1 Polyclonal antibody (28613-1-AP)

    IF Staining of HeLa using 28613-1-AP (same clone as 28613-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CDC2L1 Polyclonal antibody (28613-1-AP)

    IF Staining of HUVEC using 28613-1-AP (same clone as 28613-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28613-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CDC2L1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CDC2L1 fusion protein Ag28743
Vollständiger Name cell division cycle 2-like 1 (PITSLRE proteins)
Berechnetes Molekulargewicht 93 kDa
Beobachtetes Molekulargewicht110 kDa
GenBank-ZugangsnummerNM_001787
Gene symbol CDC2L1
Gene ID (NCBI) 984
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727)