CDK8/19 Polyklonaler Antikörper

CDK8/19 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 13761-1-AP

Synonyme

CDC2L6, CDC2 related protein kinase 6, CDC2-related protein kinase 6, CDK11, CDK19

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  WB analysis of Y79 using 13761-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDK8/19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13761-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDC2L6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13761-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDK8/19 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 13761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13761-1-AP (CDK8/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13761-1-AP (CDK8/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

13761-1-AP bindet in WB, IHC, ELISA CDK8/19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDK8/19 fusion protein Ag4731
• Vollständiger Name: cell division cycle 2-like 6 (CDK8-like)
• Berechnetes Molekulargewicht: 502 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: BC037289
• Gene symbol: CDK19
• Gene ID (NCBI): 23097
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CDK8 homolog, CDK19 (Cdk11, CDC2L6) was discovered by Hidden Markov model (HMM) profiling of a eukaryotic protein kinase domain, and found to have approximately 91% sequence homology to Cdk8. The two proteins share a particularly high degree of sequence conservation in their kinase and cyclin binding domain (PMID: 30693281, 26985305). CDK8 and CDK19, collectively termed 'Mediator Kinase,' are incorporated, in a mutually exclusive manner, as part of a 4-protein complex called the Mediator kinase module. CDK8/19 inhibition suppressed the induction of NFκB-driven transcription by p21 (PMID: 28855340).

Publikationen

Species	Application	Title
human	WB,IHC	Cell Mol Life Sci; Hotair promotes the migration and proliferation in ovarian cancer by miR-222-3p/CDK19 axis.; Authors - Lili Fan